Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
نویسندگان
چکیده
منابع مشابه
An IPTG Inducible Conditional Expression System for Mycobacteria
Conditional expression strains serve as a valuable tool to study the essentiality and to establish the vulnerability of a target under investigation in a drug discovery program. While essentiality implies an absolute requirement of a target function, vulnerability provides valuable information on the extent to which a target function needs to be depleted to achieve bacterial growth inhibition f...
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Aggregatibacter actinomycetemcomitans is the primary etiological agent of aggressive periodontal disease. Identification of novel virulence factors at the genome-wide level is hindered by lack of efficient genetic tools to perform mutagenesis in this organism. The Himar1 mariner transposon is known to yield a random distribution of insertions in an organism's genome with requirement for only a ...
متن کاملEfficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis.
A better understanding of Mycobacterium tuberculosis virulence mechanisms is highly dependent on the design of efficient mutagenesis systems. A system enabling the positive selection of insertional mutants having lost the delivery vector was developed. It uses ts-sacB vectors, which combine the counterselective properties of the sacB gene and a mycobacterial thermosensitive origin of replicatio...
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BACKGROUND Gene knockdown analyses using the in utero electroporation method have helped reveal functional aspects of genes of interest in cortical development. However, the application of this method to analyses in later stages of brain development or in the adult brain is still difficult because the amount of injected plasmids in a cell decreases along with development due to dilution by cell...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2018
ISSN: 1471-2180
DOI: 10.1186/s12866-018-1319-0